- Sequencing for IgG and IgM monoclonal antibodies
- Accurate sequencing using speciﬁc leader sequence primers
GreenLight’s platform for hybridoma sequencing can determine the DNA and amino acid sequence of your antibody. Sequencing can be performed for IgG and IgM monoclonal antibodies produced by human, mouse and rat hybridoma cell lines.
Having a 100% accurate sequence is crucial, especially if your antibody sequence is to be used for downstream activities such as recombinant antibody production or antibody humanisation. Unlike some methods that can introduce mutations at the start of Framework 1, using primers speciﬁcally designed to bind in the leader sequence, GreenLight can determine the authentic start to an antibody variable domain. This simple diﬀerence can be critical to determining the sequence of a fully functional antibody.. Determining the DNA and amino acid sequence of your preferred antibody can also form a key step to establishing patent protection for your asset.
On the extremely rare occasions where a DNA sequence cannot be obtained, as long as purified antibody is available, then peptide mapping using Mass Spec can be performed to identify the sequence of interest.
Working with GreenLight
GreenLight’s discovery services are tailored for each project to ensure that the objectives are met or exceeded. Experienced project teams are assigned to each study focusing on progressing projects through to results in the minimum amount of time. Our clients widely regard us as professional and attentive partners who deliver quality results.
To get more information, a quote or to schedule a teleconference please contact us.